Species |
Gene ID |
Accession |
Source |
Length |
MW |
Tag |
Mouse |
16163 |
P20109 |
E. coli |
111aa |
12.3kDa |
- |
About this Antibody |
Name |
Recombinant Murine IL-13 (Recombinant Murine Interleukin-13; rMuIL-13);Recombinant Mouse IL-13 ; 重組小鼠白細胞介素13 |
Synonyms |
ALRHMGC116789; BHR1interleukin-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600 |
Purity |
>97% by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4ng/ml, corresponding to a specific activity of >2.5×105IU/mg. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2μm filtered solution in PBS, pH7.4. |
Endotoxin |
Less than 1EU/μg of rMuIL-13 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
Category |
Cytokine |
Background |
Murine Interleukin-13 (IL-13) is expressed by the IL13 gene located on the chromosome 11 and secreted by many cell types, especially T helper type 2 (Th2) cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 has been implicated as a key factor in asthma, allergy, atopy and inflammatory response, establishing the protein as a valuable therapeutic target. Human, mouse and rat IL-3 share low homology, but have cross species activity. |
Amino Acid Sequence |
MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F |
包裝清單:
產品編號 |
產品名稱 |
包裝 |
P5948-2μg |
Recombinant Murine IL-13 |
2μg |
--- |
說明書 |
1份 |
保存條件:
-20℃或更低溫度保存,至少一年有效。由于蛋白的每次凍融均會引起部分失活,所以首次配制成相應濃度的儲存液后(請根據產品簡介中Reconstitution一欄的信息配制儲存液),須分裝后-20℃或更低溫度凍存,以避免反復凍融。
注意事項:
由于有些塑料管壁對某些蛋白有較強的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein,如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結合位點,使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產品簡介中Reconstitution一欄的信息配制儲存液。
本產品僅限于專業人員的科學研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內。
為了您的安全和健康,請穿實驗服并戴一次性手套操作。
使用說明:
1. 收到產品后請立即按照說明書推薦的條件保存。除非特別注明,相關產品均為凍干粉,由于微量的蛋白在凍干過程中沉積在管內,形成很薄或不可見的蛋白層,所以在打開管蓋前,我們建議在離心機中約8,000-12,000g離心10-30秒,使附著在管蓋或管壁上的蛋白聚集于管底。
2. 請根據實驗目的并按照產品簡介中Reconstitution一欄中的信息配制儲存液。大多數細胞因子或重組蛋白的凍干粉是非常容易溶解的,一般用移液槍的槍頭輕吹幾下或者輕輕搖晃瓶子,即可使細胞因子或重組蛋白完全溶解。請勿用vortex劇烈振蕩,以免蛋白變性而失活。
3. 具體的最佳工作濃度請自行參考相關文獻,或者根據實驗目的,以及特定細胞和動物,通過實驗進行摸索和優化。
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